Bioprocess Monitoring

UPSTREAM BIOPROCESSING

• Raman spectroscopy is a vital tool for upstream Process Analytical Technology (PAT), enabling the monitoring of Critical Quality Attributes (CQAs) in mammalian cell cultures. Raman-based analyzers offer an effective solution for monitoring Critical Control Parameters (CCPs) in CHO cell cultures used for monoclonal antibody production. In-line Raman immersion probes enable sterile spectral acquisition directly within the bioreactor, minimizing contamination risks associated with repeated sampling, and supporting real-time, in-line monitoring of titer, cell counts, glucose, lactate, osmolality, ammonium, and antibody concentration.

DOWNSTREAM BIOPROCESSING

• Raman spectroscopy is emerging as a technique for real-time measurements for in-line downstream process control and monitoring.
• High-throughput Raman systems are powerful tools for monitoring downstream protein purification process quality. Equipped with advanced high-throughput scanning technology, these systems generate detailed chemical information faster, with improved signal-to-noise ratios and lower limits of detection. They can be used to establish optimal purification end points, detect process excursions with root-cause determinability, enhance process understanding and control across the manufacturing process, and increase yields and profitability.

Industry Applications & ADVANTAGES OF RAMAN ANALYSIS