High-Throughput Raman Process Monitoring of Downstream Protein Purification

High-performance Raman spectroscopy, made possible by Tornado Spectral Systems’ patented High-Throughput Virtual Slit (HTVS™), enhances Raman signal by increasing photon flux by 10x to 30x as compared to conventional spectrometers. This significant advancement in technology enables faster measurements, lower limits of detection, and/or the use of intrinsically safe lower laser power, when needed. These additional gains in spectral quality and sensitivity uniquely enable success in difficult applications, such as fast measurements of proteins of interest during both upstream and downstream bioprocessing. In this white paper, learn how Tornado’s Raman analyzer is a powerful tool for monitoring downstream protein purification process quality and how it can predict the chromatographic elution concentrations of proteins (BSA and CytC) with a high degree of specificity.